objective w plan-apochromat 20×/1.0 dic m27 parfocal length (free working distance =)  (Carl Zeiss)

Journal: Nature Communications

Article Title: Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

doi: 10.1038/s41467-024-46770-2

Figure Lengend Snippet: a CAD model of the microscope with main modules labeled: excitation arms, detection path, sample stages, and sample holder. Modified or custom-made parts are red. b Size comparison between mesoSPIM v.5 and Benchtop systems. c Detection arm can be equipped with a plan apochromatic objective (2×–20× magnification, tube lens not shown) or a telecentric lens (0.9×–2×) depending on the application, with a corresponding filter wheel and set of filters. The detection arm is mounted on a focusing stage. d Comparison of sensor size, pixel size, pixel count per image, and imaging throughput between v.5 (Hamamatsu Orca Flash4.3 camera) and Benchtop (Teledyne Photometrics Iris 15). e The Benchtop mesoSPIM axial resolution in ASLM mode across the FOV, at magnification 5× and NA 0.14. The full width at half-maximum of the point-spread function along the z-axis (FWHM Z ) is color-coded from 0 to 5 µm. The resolution was measured with 0.2 µm fluorescent beads embedded in agarose immersed high-index medium (RI = 1.52). f CAD models of custom 3D printed sample holders that accommodate samples from 3 mm to 75 mm.

Article Snippet: Furthermore, the availability of Mitutoyo long-WD air parfocal objectives at 2×, 5×, 7.5×, 10×, and 20× magnifications makes this line optimal for a wide range of mesoSPIM applications.

Techniques: Microscopy, Labeling, Modification, Comparison, Imaging

Journal: Nature Communications

Article Title: Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

doi: 10.1038/s41467-024-46770-2

Figure Lengend Snippet: a The testing procedure of the detection objective contrast properties includes taking a focus stack of the Ronchi ruling immersed into the imaging medium, splitting each image into sub-regions, and calculating the local contrast of each sub-region. b Example of a 3D contrast map for objective Olympus XLFluor 4×/0.28. c Comparison of absolute contrast and field flatness across the field between Benchtop mesoSPIM (Mitutoyo Plan Apo BD 5×/0.14) vs. mesoSPIM v5 (Olympus MVPLAPO-1×, at zoom 5×). Sensor dimensions and colormap scales are equal.

Article Snippet: Furthermore, the availability of Mitutoyo long-WD air parfocal objectives at 2×, 5×, 7.5×, 10×, and 20× magnifications makes this line optimal for a wide range of mesoSPIM applications.

Techniques: Imaging, Comparison

Journal: Nature Communications

Article Title: Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

doi: 10.1038/s41467-024-46770-2

Figure Lengend Snippet: The Benchtop allows larger overall FOV and higher contrast across the image. The mesoSPIM v5 system has areas of lower resolution (ROI# 1 ) and higher resolution (ROI# 2 ) depending on the region’s position, consistent with the maps of objective contrast shown in Fig. . MIPs over multiple z-planes are shown. The original grayscale 16-bit map was pseudo-colored with a yellow LUT. Each imaging experiment was performed twice with nearly identical results.

Article Snippet: Furthermore, the availability of Mitutoyo long-WD air parfocal objectives at 2×, 5×, 7.5×, 10×, and 20× magnifications makes this line optimal for a wide range of mesoSPIM applications.

Techniques: Imaging

Journal: Nature Communications

Article Title: Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

doi: 10.1038/s41467-024-46770-2

Figure Lengend Snippet: mesoSPIM v.5 vs Benchtop comparison

Article Snippet: Furthermore, the availability of Mitutoyo long-WD air parfocal objectives at 2×, 5×, 7.5×, 10×, and 20× magnifications makes this line optimal for a wide range of mesoSPIM applications.

Techniques: